Show simple item record

dc.contributor.authorOtieno-Ayayo, Z. Ngalo
dc.contributor.authorWekesa, Euginia Makinia
dc.contributor.authorOjunga, Michaiah
dc.date.accessioned2020-07-10T11:20:59Z
dc.date.available2020-07-10T11:20:59Z
dc.date.issued2020
dc.identifier.issn2231-086X
dc.identifier.urihttp://repository.rongovarsity.ac.ke/handle/123456789/2218
dc.description.abstractAim: A study was carried out to determine the in vitro antibacterial activity of ethanol extract of root and bark of Securidaca longipedunculata against two standard isolates of Neisseria gonorrhoeae NO.0296 and WHO- K. Materials and Methods: Plant materials were collected and dried at room temperature, followed by ethanol extraction using a rotary pump. Antibacterial activity was done on the isolates using agar disc-diffusion method with Dimethyl Sulfoxide (DMSO) as a negative control and ceftriaxone as a positive control. Zone of inhibition was measured in millimeters. Minimum bactericidal concentration of the extract was determined using different concentrations of the extract then plated on Neisseria gonorrhoeae (GC) medium and a standard protocol was used to determine the presence of phytochemical compounds. Results and Discussion: The results obtained showed that both the root and bark extracts of S. longipedunculata have antibacterial activity against the two bacterial strains with a zone of inhibition of ≥ 10mm and a bactericidal activity at 0.01µg-ml of 10% crude extract. Comparison of susceptibility on tetracycline, penicillin and ciprofloxacin with the root and bark extract showed higher susceptibility to the extracts. The extracts showed presence of saponins, steroids, glycosides, flavonoids, terpenes, alkanoids, phenolics and tanins. The demonstrated antibacterial activity of S. longipedunculata against N. gonorrhoeae provides a scientific basis for the traditional use in treating venereal diseases in western Kenya. Conclusion: This investigation and further studies will pave the way for use of this plant in antibacterial drug development for alleviating human suffering. We recommend further studies to identify the specific compound(s) responsible for the antibacterial activityen_US
dc.language.isoenen_US
dc.publisherInternational Journal of Biochemistry Research & Reviewen_US
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectSecuridaca longipedunculata; phytochemicals; Neisseria gonorrhoeae.en_US
dc.titleEfficacy of Securidaca longipedunculata Fresen (Polygalaceae) against Two Standard Isolates of Neisseria gonorrhoeaeen_US
dc.typeArticleen_US


Files in this item

Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-ShareAlike 3.0 United States
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 3.0 United States